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KMID : 1234420100380030295
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 3 p.295 ~ p.301
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Selection and Mechanisms of Indigenous Antagonistic Microorganisms against Sheath Rot and Dry Rot Disease of Garlic
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Jeong Hee-Young
Lim Jong-Hui
Kim Byung-Keuk
Lee Jung-Jong
Kim Sang-Dal
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Abstract
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Sheath rot and dry rot disease caused by Pseudomonas marginalis and Fusarium oxysporum were serious problems in
garlic farmland. In this study, total of 160 indigenous antagonistic bacteria were isolated from 16 farmlands in Yeongcheon, Korea. Among these, 15 strains were able to inhibited P. marginalis and F. oxysporum. The 16s rDNA genes of the selected 15 strains were amplified and sequenced. The strains has strong antagonistic ability against garlic pathogens was achieved Bacillus subtilis YC82, B. vallismortis YC84, B. amyloliquefaciens YC240. The selected 3 strains tested for investigation of antifungal mechanisms further analyses; 3 strains of these validated for production of siderophore, β-glucanase and chitinase using CAS (chrome azurol S) blue agar, CMC-congo red agar and DNS method. The 3 strains were able to utilized insoluble phosphate as dertermined by vanado-molybdate method. The 3 strains verified for production of auxin and gibberellic acid using Salkowski test and holdbrook test. Also, 3 strains showed stimulation germination, stem growth promoting activity on the in vivo test. The 3 strains were able to effectively suppress P. marginalis and F. oxysporum causing sheath rot and dry rot diseases on the in vivo pot test.
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KEYWORD
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sheath rot, dry rot, garlic, indigenous antagonistic microorganism
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